minimum essential medium alpha (amem (Thermo Fisher)

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Thermo Fisher minimum essential medium alpha (amem
Minimum Essential Medium Alpha (Amem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alpha+minimum+essential+medium+amem/pmc11681832-75-11-23
Average 90 stars, based on 1 article reviews

minimum essential medium alpha (amem - by Bioz Stars, 2026-09

90/100 stars

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Suspension:

Article Title: A diazirine-based photoaffinity etoposide probe for labeling topoisomerase II
Article Snippet: K562 cells were obtained from American Type Culture Collection (Rockville, MD). .. K/VP.5 cells are a 26-fold etoposide-resistant K562-derived sub-line with decreased levels of topoisomerase IIa mRNA and protein.40 These cells were maintained as suspension cultures in alpha minimum essential medium (aMEM) (Gibco BRL, Burlington, Canada) containing 2 mM L-glutamine and supplemented with 10% fetal calf serum (Invitrogen, Burlington, ON, Canada), 20 mM NaHCO3, 20 mM HEPES (Sigma), 100 units/ml penicillin G, and 100 lg/ml streptomycin at pH 7.4 in an atmosphere of 5% CO2 and 95% air at 37 C. For the measurement of growth inhibition, cells in exponential growth were harvested and seeded at 6000 cells/well in 96-well plates (100 ll/well). ..

Inhibition:

Generated:

Article Title: Mechanism of mesenchymal stem cell-induced neuron recovery and anti-inflammation.
Article Snippet: Background aims.. After ischemic or hemorrhagic stroke, neurons in the penumbra surrounding regions of irreversible injury are vulnerable to delayed but progressive damage as a result of ischemia and hemin-induced neurotoxicity.. There is no effective treatment to rescue such dying neurons.

Cell Culture:

Article Title: Influence of shaking culture on the biological functions of cell aggregates incorporating gelatin hydrogel microspheres.
Article Snippet: Laboratory of Biomaterials, Institute for Frontier Life and Medical Sciences, Kyoto University, 53 Kawara-cho Shogoin, Sakyo-ku, Kyoto 606-8507, Japan, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda 278-8510, Japan, Center for Drug Delivery Research, Tokyo University of Science, 2641 Yamazaki, Noda 278-8510, Japan, and Center for Physical Pharmaceutics, Tokyo University of Sciences, 2641 Yamazaki, Noda 278-8510, Japan

Article Title: Evaluation of the In Vitro Cytotoxicity of Crosslinked Biomaterials
Article Snippet: L929 cells were cultured per the manufacturer’s specifications with Minimum Essential Medium (αMEM) (Life Technologies, Frederick, MD) and 10% horse serum (Life Technologies). .. MC3T3, a mouse osteoblast precursor cell, were cultured per the manufacturer’s specifications with alpha Minimum Essential Medium (aMEM) (Life Technologies) containing ribonucleosides, deoxyribonucleosides, 2 mM L-glutamine (Life Technologies), 1 mM sodium pyruvate (Life Technologies), and 10% fetal bovine serum (FBS) (Life Technologies). .. The hMSCs were cultured as previously described and per the manufacturer’s protocol, with Dulbeccos Modified Eagle Medium (DMEM) (Life Technologies), supplemented with 10% fetal bovine serum (Life Technologies), 1.0% v/v penicillin/streptomycin (Life Technologies), 0.1mM non-essential amino acids (Life Technologies), and 4mM L-glutamine (Life Technologies) 18 , 19 .

Derivative Assay:

Real-time Polymerase Chain Reaction:

Article Title: Matrix protein biglycan induces osteoblast differentiation through extracellular signal-regulated kinase and Smad pathways.
Article Snippet: .. MC3T3-E1 cells were maintained in alpha minimum essential medium (aMEM) (Gibco, U.S.A.) supplemented with 10% fetal bovine serum (FBS), 100 units/ml penicillin, and 100 mg/ml streptomycin. mRNA Assay RNA was extracted from cells using Trizol (Invitrogen, U.S.A.). cDNA was made with SuperScript III (Invitrogen, U.S.A.) using oligo dTs as primers. cDNA was subsequently used for Quantitative real-time polymerase chain reaction (PCR) (RT-qPCR) with SYBR Green (Toyobo, Japan). ..

Polymerase Chain Reaction:

SYBR Green Assay:

Comparison:

Article Title: Direct comparison of current cell-based and cell-free approaches towards the repair of craniofacial bone defects - A preclinical study.
Article Snippet: http://dx.doi.org/10.1016/j.actbio.2015.08.013 1742-7061/ 2015 Published by Elsevier Ltd. on behalf of Acta Materialia Inc. ⇑ Corresponding author at: Clinique de Stomatologie et de Chirurgie maxillofaciale, Centre Hospitalier Universitaire de Nantes, 1 place Alexis Ricordeau, 44093 Nantes Cedex 1, France.. E-mail address: pierre.corre@chu-nantes.fr (P. Corre).. 1 These authors contributed equally to the work acting as co-first or co-last authors.

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